Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1224360 | Journal of Pharmaceutical and Biomedical Analysis | 2007 | 5 Pages |
Abstract
A liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for the determination of azelnidipine in human plasma was established. Nicardipine was used as the internal standard (IS). After adjustment to a basic pH with sodium hydroxide solution (0.1Â M), plasma samples were extracted with cyclohexane-diethyl ether (1:1, v/v) and separated on a C18 column with a mobile phase of 20Â mM ammonium acetate solution-methanol-formic acid (25:75:0.5, v/v). The electrospray ionization was employed in a single quadrupole mass spectrometer for the determination. The method was linear over the concentration range of 0.05-40Â ng/ml. The lower limit of quantification (LLOQ) was 0.05Â ng/ml. The intra- and inter-run standard deviations were less than 9.5% and 11.0%, respectively. The method was successfully applied to study the pharmacokinetics of azelnidipine in healthy Chinese volunteers.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Li Ding, Li Li, Pengcheng Ma,