Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1224437 | Journal of Pharmaceutical and Biomedical Analysis | 2006 | 8 Pages |
Abstract
A stereospecific HPLC methodology has been developed for the diastereoisomeric resolution of a mononucleotide prodrug in cell extracts. This method involves the use of solid phase extraction on a C18 cartridge. Diastereoisomers and internal standard resolutions were performed on a cellulose based chiral column (Chiralcel OD-H) used in the normal phase mode. The method was validated in terms of specificity, recovery, linearity (diasteroisomers mixture concentration: 3-60 μmol Lâ1), precision and accuracy and detection limit (1.67 and 1.33 μmol Lâ1 for first and second eluted diastereoisomer). This method was applied to the determination of the apparent rate constants of disappearance and half-lives of each stereoisomers. This permits to conclude to the stereoselectivity of the enzymatic activity involved in the decomposition pathway of 2.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
C. Foulon, C. Vaccher, A.-L. Villard, J.-Y. Puy, I. Lefebvre, C. Perigaud, J.P. Bonte, J.F. Goossens,