Article ID Journal Published Year Pages File Type
1224460 Journal of Pharmaceutical and Biomedical Analysis 2007 8 Pages PDF
Abstract

Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was operated in positive mode (PI) to characterize polymethoxylated flavonoids (PMFs) through its specific radical cations by collision-induced dissociation (CID). The fragments of [M + H − n × 15]+ produced by loss of one or more methyl group from the protonated molecule, as well as [M + H − 29]+, [M + H − 31]+, [M + H − 33]+, [M + H − 43]+, [M + H − 46]+, and [M + H − 61]+ fragment ions were detected, which were diagnostic for the polymethoxylated species, and could be adopted to form the multiple MS (MSn) “fingerprint” of PMFs. Based on this “fingerprint”, 29 PMFs were screened out from extracts of Fructus aurantii, among which two of them were identified as sinensetin and tangeretin. It was proved that the PI was suitable for structural characterization of PMFs by APCI-MSn.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , , ,