| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1224650 | Journal of Pharmaceutical and Biomedical Analysis | 2007 | 5 Pages |
Abstract
In order to investigate the enantiomeric pharmacokinetics and biotransformation of synthetic anisodamine (654-2), a cholinoceptor antagonist widely used in clinic in China, it has been preparatively separated into two racemates (I and II) by using ZORBAX Eclipse XDB-C18 column. The diastereo- and/or enantioseparations of 654-2, I and II were carried out by HPLC using CHIRALPAK AD-H as chiral stationary phase (CSP) and acetonitrile-2-propanol-DEA 97:3:0.1 (v/v/v) as mobile phase. The methods were optimized by studying mobile phase modifiers, concentration of modifier and column temperature. The HPLC method for the simultaneous separation of two pairs of enantiomers of 654-2 has been validated.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Li-Min Yang, Yi-Fan Xie, Hong-Zhuan Chen, Yang Lu,