Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1224965 | Journal of Pharmaceutical and Biomedical Analysis | 2006 | 4 Pages |
In this paper, the chiral separation of cefadroxil was studied by capillary electrochromatography. Monolithic capillary column was prepared for the separation of cefadroxil enantiomers. The optimum buffer contained 28.5 mmol/L sodium acetate, 0.95% (v/v) acetic acid, 19 mmol/L β-cyclodextrin (β-CD) and 5% (v/v) isopropanol in formamide solution (pH 7.0), with the running voltage of 12 kV, the UV detector wavelength of 254 nm, the sample injected time of 8 s and the temperature of 25 °C. Under these conditions, the column efficiency of cefadroxil enantiomers were N1 = 5324 and N2 = 23,768 with a selectivity factor (α) of 1.056 and resolution (Rs) of 0.978. The effect of buffer pH value, β-CD concentration, organic modifier (isopropanol) concentration and voltage was also investigated for the separation by CEC.