Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1224972 | Journal of Pharmaceutical and Biomedical Analysis | 2006 | 6 Pages |
Abstract
The interaction of metronidazole (MTZ) and human serum albumin (HSA) was studied using the coupling system of on-line microdialysis sampling with flow-injection chemiluminescence detection (FI-MD-CL). The interested drug and HSA were mixed in different molar ratios in 0.067 mol Lâ1 phosphate buffer (pH 7.4) and incubated at 37 °C in a water-bath. Then the microdialysis probe was put into the MTZ-HSA mixed solution and sampled at a perfusion rate of 5 μL minâ1. The microdialysates was determined using flow-injection chemiluminescence. In vitro recovery (R) of MTZ under experimental conditions was approximately 25.2% with a R.S.D. of about 3.2%. The values estimated for the binding constant (K) and the number of the binding sites (n) were found to be 1.50 Ã 103 L molâ1 and 1.89, respectively. The values of nK obtained using Scatchard analysis and Klotz plot were found to be quite similar. The method provided a reliable and simple technique for the study of drug-protein interaction.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hua Chen, Zhengjun Gong, Zhujun Zhang,