Article ID Journal Published Year Pages File Type
1224982 Journal of Pharmaceutical and Biomedical Analysis 2006 5 Pages PDF
Abstract
A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (λex = 305 nm; λem = 390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL−1 in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL−1. This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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