Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1224983 | Journal of Pharmaceutical and Biomedical Analysis | 2006 | 5 Pages |
Capillary zone electrophoresis with spectrophotometric detection was used for the determination of ibuprofen (IB) and flurbiprofen (FL) in pharmaceuticals. The separation was carried out in a fused silica capillary (60 cm × 100 μm i.d. effective length 45 cm) at 30 kV with UV detection at 232 nm. The optimized background electrolyte was 20 mM N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) with 20 mM imidazole and 10 mM α-cyclodextrin of pH 7.3. 2-Naphthoxyacetic acid was used as internal standard. A single analysis took less than 5 min. Rectilinear calibration ranges were 2–500 mg l−1 for IB and 1–60 mg l−1 for FL. The relative standard deviations (R.S.D.) values (n = 6) were 1.53% for IB and 1.29% for FL (for 200 mg l−1 IB and 10 mg l−1 FL). This validated method has been successfully applied for the routine analysis of 10 commercially available pharmaceutical preparations (syrup, tablets, cream and gel).