Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1225027 | Journal of Pharmaceutical and Biomedical Analysis | 2006 | 5 Pages |
Abstract
A high-performance liquid chromatography method using ultraviolet detection at 230 nm for the simultaneous determination of amoxicillin and ranitidine in rat plasma has been validated. Plasma samples after pretreatment with acetonitrile to effect deproteinization were dried under N2 and reconstituted with water. The standard calibration curves for amoxicillin and ranitidine were linear (r2 = 0.9999) over the concentration range of 0.2-20 μg mlâ1 and 0.03-6 μg mlâ1 in rat plasma, respectively. The intra- and inter-day assay variability range for amoxicillin was 2.4-8.5% and 3.2-11.7%, and for ranitidine was 1.7-9.0% and 4.5-10.1%, respectively. This method has been successfully applied to a pharmacokinetic study after oral coadministration of amoxicillin and ranitidine to rats.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Li, Fengping Tan, Kang Zhao,