| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1225060 | Journal of Pharmaceutical and Biomedical Analysis | 2006 | 7 Pages |
Abstract
A novel method for analysis of hydrophobic drug molecules in matrices that contain Cremophor® EL (CrEL) is presented. The method utilized a precipitation technique involving mercuric chloride in a reaction with CrEL to form an insoluble complex in an ethanol matrix. The hydrophobic drug molecule was then analyzed by HPLC-UV without interference from CrEL. Nuclear magnetic resonance and infrared spectroscopy indicated that the mechanism of precipitation involves the reaction of mercuric chloride with the ether bond of CrEL. Analysis by HPLC with UV detection of paclitaxel and related substances was used to verify that the reaction is specific toward CrEL.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
James D. Perdue, Pamela J. Seaton, John A. Tyrell, Daniel R. DeVido,