| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1225450 | Journal of Proteomics | 2015 | 12 Pages |
•Urinary tract infections and dog as reservoir and animal model•E. coli isolates from UTI and antibiotic resistance to enrofloxacin•Differential protein expression of enrofloxacin-induced E. coli isolate•Resistance mechanisms involve membrane permeability and stabilization of DNA.
Escherichia coli (E. coli) urinary tract infections (UTIs) are becoming a serious problem both for pets and humans (zoonosis) due to the close contact and to the increasing resistance to antibiotics.This study has been performed in order to unravel the mechanism of induced enrofloxacin resistance in canine E. coli isolates that represent a good tool to study this pathology.The isolated E. coli has been induced with enrofloxacin and studied through 2D DIGE and shotgun MS.Discovered differentially expressed proteins are principally involved in antibiotic resistance and linked to oxidative stress response, to DNA protection and to membrane permeability. Moreover, since enrofloxacin is an inhibitor of DNA gyrase, the overexpression of DNA starvation/stationary phase protection protein (Dsp) could be a central point to discover the mechanism of this clone to counteract the effects of enrofloxacin. In parallel, the dramatic decrease of the synthesis of the outer membrane protein W, which represents one of the main gates for enrofloxacin entrance, could explain additional mechanism of E. coli defense against this antibiotic. All 2D DIGE and MS data have been deposited into the ProteomeXchange Consortium with identifier PXD002000 and DOI http://dx.doi.org/10.6019/PXD002000.This article is part of a Special Issue entitled: HUPO 2014.
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