| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1225588 | Journal of Proteomics | 2013 | 20 Pages |
Butanol is a promising biofuel, and recent metabolic engineering efforts have demonstrated the use of photosynthetic cyanobacterial hosts for its production. However, cyanobacteria have very low tolerance to butanol, limiting the economic viability of butanol production from these renewable producing systems. The existing knowledge of molecular mechanism involved in butanol tolerance in cyanobacteria is very limited. To build a foundation necessary to engineer robust butanol-producing cyanobacterial hosts, in this study, the responses of Synechocystis PCC 6803 to butanol were investigated using a quantitative proteomics approach with iTRAQ — LC-MS/MS technologies. The resulting high-quality dataset consisted of 25,347 peptides corresponding to 1452 unique proteins, a coverage of approximately 40% of the predicted proteins in Synechocystis. Comparative quantification of protein abundances led to the identification of 303 differentially regulated proteins by butanol. Annotation and GO term enrichment analysis showed that multiple biological processes were regulated, suggesting that Synechocystis probably employed multiple and synergistic resistance mechanisms in dealing with butanol stress. Notably, the analysis revealed the induction of heat-shock protein and transporters, along with modification of cell membrane and envelope were the major protection mechanisms against butanol. A conceptual cellular model of Synechocystis PCC 6803 responses to butanol stress was constructed to illustrate the putative molecular mechanisms employed to defend against butanol stress.
Graphical abstractThe responses of Synechocystis PCC 6803 to butanol were investigated using a quantitative proteomics approach with iTRAQ — LC-MS/MS technologies. The results showed that Synechocystis employed multiple and synergistic resistance mechanisms in dealing with butanol stress.Figure optionsDownload full-size imageDownload high-quality image (421 K)Download as PowerPoint slideHighlights► A quantitative proteomics used to investigate butanol resistance in Synechocystis. ► Synechocystis employs multiple mechanisms in coping with butanol stress. ► Putative resistance mechanisms against butanol in Synechocystis were proposed. ► A list of potential targets for engineering butanol resistance was provided.
