| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1225625 | Journal of Proteomics | 2012 | 13 Pages |
The soy-derived phytoestrogen genistein (GEN) has received attention for its potential benefits on the cardiovascular system by providing direct protection to cardiomyocytes against pathophysiological stresses. Here, we employed a proteomic approach to study the concentration-dependent effects of GEN treatments on cardiomyocytes. Cultured HL-1 cardiomyocytes were treated with low (1 μM) and high (50 μM) concentrations of GEN. Proteins were pre-fractionated by sequential hydrophilic/hydrophobic extraction and both protein fractions from each treatment group were separated by 2D gel electrophoresis (2DE). Overall, approximately 2,700 spots were visualized on the 2D gels. Thirty-nine and 99 spots changed in volume relative to controls (p < 0.05) following the low- and high-concentration GEN treatments, respectively. From these spots, 25 and 62 protein species were identified by ESI-MS/MS and Mascot database searching, respectively. Identified proteins were further categorized according to their functions and possible links to cardioprotection were discussed. MetaCore gene ontology analysis suggested that 1 μM GEN significantly impacted the anti-apoptosis process, and that both the low and high concentrations of GEN influenced the glucose catabolic process and regulation of ATPase activity. This proteomics study provides the first global insight into the molecular events triggered by GEN treatment in cardiomyocytes.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (376 K)Download as PowerPoint slideHighlights►Significant concentration-dependent effects were observed using proteomic analysis of genistein-exposed cardiomyocytes. ►The two-stage sequential extraction approach enabled visualization of more than 2500 spots in 2DE. ►Up-regulation of cytoprotective genes was observed in cardiomyocytes exposed to 1 μM genistein. ►Down-regulation of glycolytic and antioxidant genes was observed in cardiomyocytes exposed to 50 μM genistein.
