| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1225810 | Journal of Proteomics | 2011 | 13 Pages |
Iron is essential for all living organisms and plays a crucial role in pathogenicity. This study presents the first proteome analysis of plasma membranes isolated from pea roots. Protein profiles of four different samples (+ Fe, + Fe/Chitosan, − Fe, and − Fe/Chitosan) were compared by native IEF-PAGE combined with in-gel activity stains and DIGE. Using DIGE, 89 proteins of interest were detected in plasma membrane fractions. Data revealed a differential abundance of several spots in all samples investigated. In comparison to the control and − FeCh the abundance of six protein spots increased whereas 56 spots decreased in + FeCh. Altered protein spots were analyzed by MALDI-TOF-TOF mass spectrometry. Besides stress-related proteins, transport proteins and redox enzymes were identified. Activity stains after native PAGE and spectrophotometric measurements demonstrated induction of a ferric-chelate reductase (− Fe) and a putative respiratory burst oxidase homolog (− FeCh). However, the activity of the ferric-chelate reductase decreased in − Fe plants after elicitor treatment. The activity of plasma membrane-bound class III peroxidases increased after elicitor treatment and decreased under iron-deficiency, whereas activity of quinone reductases decreased mostly after elicitor treatment. Possible functions of proteins identified and reasons for a weakened pathogen response of iron-deficient plants were discussed.
Graphical abstractIron-deficient pea plants appear more susceptible to pathogen infection. The molecular mechanisms and the function of plasma membrane-bound proteins involved in these reactions are not very well understood. In the present study the plasma membrane proteome of pea grown in the presence or absence of iron (+ Fe, − Fe) and after elicitation with Chitosan (+ Fe/Chitosan, − Fe/Chitosan) were compared by native IEF-PAGE combined with in-gel activity stains and DIGE.Figure optionsDownload full-size imageDownload high-quality image (32 K)Download as PowerPoint slide
