Article ID Journal Published Year Pages File Type
1225872 Journal of Proteomics 2016 7 Pages PDF
Abstract

•Differences in urine protein composition due to genetic variability are a main problem for defining biomarkers•Large monogenic pedigrees with reduced genetic background are potentially good models to characterise urinary biomarkers in selected disease•Characterizing a large family with Dent's disease led to definition of specific biomarkers.•Gene ontology analysis revealed high urinary levels of at least 15 protein clusters.•Proteins involved in extracellular matrix remodelling was a major group of interest.

Definition of the urinary protein composition would represent a potential tool for diagnosis in many clinical conditions. The use of new proteomic technologies allows detection of genetic and post-trasductional variants that increase sensitivity of the approach but complicates comparison within a heterogeneous patient population. Overall, this limits research of urinary biomarkers. Studying monogenic diseases are useful models to address this issue since genetic variability is reduced among first- and second-degree relatives of the same family.We applied this concept to Dent's disease, a monogenic condition characterised by low-molecular-weight proteinuria that is inherited following an X-linked trait. Results are presented here on a combined proteomic approach (LC-mass spectrometry, Western blot and zymograms for proteases and inhibitors) to characterise urine proteins in a large family (18 members, 6 hemizygous patients, 6 carrier females, and 6 normals) with Dent's diseases due to the 1070G > T mutation of the CLCN5.Gene ontology analysis on more than 1000 proteins showed that several clusters of proteins characterised urine of affected patients compared to carrier females and normal subjects: proteins involved in extracellular matrix remodelling were the major group. Specific analysis on metalloproteases and their inhibitors underscored unexpected mechanisms potentially involved in renal fibrosis.Biological significanceStudying with new-generation techniques for proteomic analysis of the members of a large family with Dent's disease sharing the same molecular defect allowed highly repetitive results that justify conclusions. Identification in urine of proteins actively involved in interstitial matrix remodelling poses the question of active anti-fibrotic drugs in Dent's patients.

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Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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