Article ID Journal Published Year Pages File Type
1225891 Journal of Proteomics 2016 10 Pages PDF
Abstract

•We successfully identified 713 unique proteins in ES proteins from 14 day schistosomula of S. japonicum.•Bioinformatics analysis revealed that identified ES proteins were mainly associated with diverse biological processes.•Recombinant SjTPx can inhibit the expression of surface markers (MHC II and CD86) on LPS-activated macrophages.

Schistosomiasis remains a serious public health problem, with 200 million people infected and 779 million people at risk worldwide. The schistosomulum is the early stage of the complex lifecycle of Schistosoma japonicum in their vertebrate hosts, and is the main target of vaccine-induced protective immunity. Excretory/secretory (ES) proteins play a major role in host–parasite interactions and ES protein compositions of schistosomula of S. japonicum have not been characterized to date. In the present study, the proteome of ES proteins from 14 day schistosomula of S. japonicum was analyzed by liquid chromatography/tandem mass spectrometry and 713 unique proteins were finally identified. Gene ontology and pathway analysis revealed that identified proteins were mainly involved in carbohydrate metabolism, degradation, response to stimulus, oxidation–reduction, biological regulation and binding. Flow cytometry analysis demonstrated that thioredoxin peroxidase identified in this study had the effect on inhibiting MHCII and CD86 expression on LPS-activated macrophages. The present study provides insight into the growth and development of the schistosome in the final host and valuable information for screening vaccine candidates for schistosomiasis.

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Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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