Physiological response and differential leaf proteome pattern in the European invasive Asteraceae Solidago canadensis colonizing a former cokery soil

Derelict contaminated sites are often colonized spontaneously by plant species leading to a vegetal cover thought to limit particle dispersal and polluted water infiltration. Those plants must cope with soil pollutants through tolerance mechanisms that are not yet fully understood. Here, we focused our attention on a particular Asteraceae plant, Solidago canadensis, considered as invasive in Europe. S. canadensis spontaneously growing on either polluted (NM soil) or control soils dumped on experimental plots were studied for their physiological status, oxidative stress and 2D-DIGE of leaf extracts. S. canadensis tolerance to soil pollutants was demonstrated since growth rates, allocation to reproduction ratios and Fv/Fm ratios were similar in plants from control and NM soil. At the cell level, the catalase activity level was increased in plants collected on NM soil while lipoperoxidation was unaffected. Also, the leaf proteomic study revealed thirty down-regulated and sixty-six up-regulated proteins. Abundances of proteins related to oxidative stress, carbohydrate metabolism, ion transport were mainly up-regulated while those of proteins involved in cell cycle and transcription/translation were mostly down-regulated. Proteins associated to protein metabolism were either down- or up-regulated. Considered altogether, we highlighted that S. canadensis exhibited a complex proteome response when experiencing a multicontaminated soil.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (67 K)Download as PowerPoint slideHighlights► For the first time we report on the leaf proteome of an invasive plant species Solidago canadensis. ► S. canadensis grown on control and multicontaminated cokery soils were compared. ► S. canadensis tolerated the polluted soil as demonstrated by unaltered growth rate and PSII fluorescence measurements. ► In pollutant-exposed plants, proteomics showed up- and down regulation of several key metabolic pathways.