| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1227096 | Journal of Proteomics | 2011 | 10 Pages |
Chagas disease has a variable clinical course with different manifestations and heterogenous geographical distribution. Some studies suggest that this clinical variability could be influenced by the genetic variability of T. cruzi. Here we present the differential protein expression among trypomastigotes and amastigotes of T. cruzi group I isolates from patients with acute and chronic form of Chagas disease from Santander, Colombia. A total of 29 proteins were identified by MALDI-TOF and LC-MS/MS; twenty in trypomastigote and nine in amastigote stage. The 29 proteins identified were grouped in 7 functional categories: 1) metabolism 31%, 2) assembly of cytoskeleton 13.7%, 3) protein destination 13.7%, 4) defenses antioxidants 20.6%, 5) protein synthesis and cellular cycle 13.7%, 6) catabolism 6.8%, and 7) adhesion 3.4%. Tryparedoxin peroxidase, lipoamide dehydrogenase, tyrosine amino transferase and HSP70 were overexpressed in the acute Chagas isolate. Tryparedoxin peroxidase overexpression in the acute isolate was confirmed by Western blot analysis. Most of these proteins are associated with resistance to oxidative stress facilitating their survival within host cells. Therefore, these proteins may represent virulence factors associated with the development of the acute form of the disease and could be used as biomarkers of the clinical course of disease and as drug targets.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (53 K)Download as PowerPoint slideResearch highlights► We analyzed the differential protein expression of T. cruzi I isolates from patients with acute and chronic Chagas disease. ► The function of the most differentially expressed proteins were metabolism, defenses antioxidants and assembly of cytoskeleton. ► The proteins overexpressed in the acute isolate are associated with resistance to oxidative stress related with parasite survival. ► These proteins may represent virulence factors and could be used as clinical biomarkers and as drug targets.
