Influence of Cu10x copper nanoparticles intramuscular injection on mineral composition of rat spleen

Spleen mineral composition was studied for 25 elements using atomic emission and mass spectrometry (AES-ISP and MS-ISP) for the first time by one- and manifold intramuscular injection of Cu10x copper nanoparticles. This was done, because the spleen is considered to be the organ most amenable of immunological defense and sensitive to exposure. The research is new and based on the necessity to check the accumulation of elements, which can result in toxic effects, and also the character of the influence of nanoparticles on the change of the natural fluctuation rhythm, as well as the interrelation between them.