Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat

Biosynthesis of AgNPs by 37 different bacterial soil isolates was done and confirmed through visible spectrophotometry. Fifteen isolates were identified and two of them with the highest ability of AgNPs production were used for Transmission Electron Microscopy (TEM) and X-ray diffraction (XRD) tests. MTT assay for both of the obtained AgNPs was run and after determination their IC50s, two different toxic and nontoxic doses of each AgNPs solution were chosen for wound healing assay. Forty eight rats were divided into 6 groups; two were the controls, two were administrated by the toxic and two were administrated by the nontoxic doses of AgNPs produced by Bacillus cereus and Escherichia fergusonii. Administration of the nontoxic doses of AgNPs had better wound healing effect than both of the toxic ones. The control groups had less wound healing properties. In conclusion, biologically produced AgNPs in their nontoxic doses accelerated the collagen formation and the epithelization and decelerated the angiogenesis and duration of completion the epithelization.