Aptamers as the ‘capturing’ agents in aptamer-based capture assays

•Immobilization strategies of aptamers.•Different target purification techniques.•Applications of AptaCapture assays.

Aptamers are widely used as diagnostic agents due to their specificity and high binding affinity to their cognate target. Apart from diagnostic applications, aptamers can also be employed to capture their cognate target. Aptamer-based capture (AptaCapture) assays enable capture of the target by the aptamers followed by target purification. This review focuses on the immobilization strategies of aptamers, different target purification techniques, and applications of AptaCapture assays.