Article ID Journal Published Year Pages File Type
1227554 Microchemical Journal 2016 5 Pages PDF
Abstract

•GCE/MWCNTs-Cyt c was successfully used as bioanalytical platform to design a bienzymatic biosensor.•The intimate interaction of Cyt c with MWCNTs and GOx facilitates the biocatalytic detection of glucose.•The use of hydroquinone as redox mediator allowed the sensitive quantification of glucose.•GCE/MWCNTs-Cyt c/GOx/Naf was successfully used for the quantification of glucose in real samples.

We report for the first time the use of glassy carbon electrodes (GCE) modified by drop-coating with multi-walled carbon nanotubes (MWCNTs) dispersed in cytochrome c as platform for the construction of bienzymatic biosensors, using the glycoenzyme glucose oxidase (GOx) as model. The sensitivity of the resulting biosensor at − 0.050 V towards glucose using hydroquinone (H2Q) as redox mediator was (96 ± 2) μA M− 1, with a linear range between 1.00 × 10− 4 M and 1.00 × 10− 3 M and a detection limit of 8 μM. The biosensor demonstrated to be highly reproducible with RSD. values of 6.1% for five different bioelectrodes. The combination of the excellent dispersing properties of Cyt c, the stability of MWCNTs-Cyt c dispersion and the intimate interaction between Cyt c, MWCNTs and GOx, allowed the construction of an efficient glucose biosensor successfully used to determine glucose in commercial beverages.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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