Mean centering of double divisor ratio spectra, a novel spectrophotometric method for analysis of ternary mixtures

•Novel spectrophotometric method for analysis of ternary mixtures•Simple calculation instead of multi-step Mean Centering method•Sensitive method compared to double divisor of ratio spectra method•Real samples analyzed without spiking of double divisor components•Spectrophotometric determination of Amlodipine, Aliskiren and Hydrochlorothiazide in dosage form

A novel spectrophotometric method was developed for determination of ternary mixtures without previous separation, showing significant advantages over conventional methods. The new method is based on mean centering of double divisor ratio spectra. The mathematical explanation of the procedure is illustrated. The method was evaluated by determination of model ternary mixture and by the determination of Amlodipine (AML), Aliskiren (ALI) and Hydrochlorothiazide (HCT) in laboratory prepared mixtures and in a commercial pharmaceutical preparation. For proper presentation of the advantages and applicability of the new method, a comparative study was established between the new mean centering of double divisor ratio spectra (MCDD) and two similar methods used for analysis of ternary mixtures, namely mean centering (MC) and double divisor of ratio spectra-derivative spectrophotometry (DDRS-DS). The method was also compared with a reported one for analysis of the pharmaceutical preparation. The method was validated according to the ICH guidelines and accuracy, precision, repeatability and robustness were found to be within the acceptable limits.

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