| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1228810 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2015 | 7 Pages |
•Enhancement of absorbance and fluorescence intensity occurs together with complex formation of INS with β-CDx.•BH plot confirms the 1:1 stoichiometric ratio.•Lifetime analysis confirms the complex between INS and β-CD in liquid state.•FT-IR, SEM, DSC and XRD analysis confirms the complex formation between INS and β-CDx in solid state.•The orientation of the complex is confirmed by molecular docking study.
Inosine is a nucleoside that is formed when hypoxanthine is attached to a ribose ring (also known as a ribofuranose) via a β-N9-glycosidic bond. Inosine is commonly found in tRNAs. Inosine (INS) has been used widely as an antiviral drug. The inclusion complex of INS with β-CDx in solution phase is studied by ground and excited state with UV–visible and fluorescence spectroscopy, respectively. A binding constant and stoichiometric ratio between INS and β-CDx are calculated by BH equation. The lifetime and relative amplitude of INS is increases with increasing the concentrations of β-CDx, confirms the formation of inclusion complex in liquid state. The solid complexes are prepared by kneading method (KM) and co-precipitation method (CP). The solid complex is characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), powder X-ray diffraction (XRD) and differential scanning colorimetry (DSC). CP method gives the solid product with good yield than that of physical mixture and KM method. The structure of complex is proposed based on the study of Patch – Dock server.
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