| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1228850 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2016 | 6 Pages |
•The cytotoxicity of complexes 1 and 2 against six cell lines was studied.•The apoptosis and cellular uptake in BEL-7402 cells were investigated.•The levels of ROS and the mitochondrial membrane potential were assayed.•The cell cycle arrest was investigated by flow cytometry.•The BSA-binding behaviors of the complexes were studied.
The cytotoxic activity of two Ru(II) complexes against A549, BEL-7402, HeLa, PC-12, SGC-7901 and SiHa cell lines was investigated by MTT method. Complexes 1 and 2 show moderate cytotoxicity toward BEL-7402 cells with an IC50 value of 53.9 ± 3.4 and 39.3 ± 2.1 μM. The effects of the complexes inducing apoptosis, cellular uptake, reactive oxygen species and mitochondrial membrane potential in BEL-7402 cells have been studied by fluorescence microscopy. The percentages of apoptotic and necrotic cells and cell cycle arrest were studied by flow cytometry. The BSA-binding behaviors were investigated by UV/visible and fluorescent spectra.
Graphical abstractThe cytotoxicity in vitro, apoptosis, ROS, mitochondrial membrane potential, cell cycle arrest induced by the two Ru(II) complex were investigated.Figure optionsDownload full-size imageDownload as PowerPoint slide
