| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1229103 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2015 | 9 Pages |
•Photophysical properties of 3,6-diHF in CDs/BSA systems, were studied.•The excited-state intramolecular proton transfer depends of the CDs cavity and BSA.•The intrinsic fluorescence of BSA in the 3,6-diHF/BSA/CDs systems, is discussed.•The use of 3,6-diHF as sensitive fluorescence probe in biological systems.
Photophysical properties of a bioactive flavonol which can be used as a model for polyhydroxylated natural flavonols, 3,6-diHydroxyflavone (3,6-diHF) in cyclodextrins (CDs)/bovine serum albumin (BSA) systems have been studied by absorption and fluorescence spectroscopy. The influence of CDs nature and of the different molar ratios BSA/CDs on the fluorescent characteristics of 3,6-diHF, and on the excited – state intramolecular proton transfer (ESIPT) process were studied. Quantitative information on the interaction between 3,6-diHF and BSA in CDs medium, were estimated. The influence of temperature (25–60 °C range) on the intrinsic fluorescence of BSA in 3,6-diHF/BSA/CDs systems, was investigated. The results are discussed with relevance to 3,6-diHF as a potential sensitive fluorescence probe in the systems of biological interest.
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