Spectrophotometric and chemometric methods for determination of imipenem, ciprofloxacin hydrochloride, dexamethasone sodium phosphate, paracetamol and cilastatin sodium in human urine

•Spectrophotometric and chemometric methods were developed and validated.•Principal component regression and partial least-squares done for this mixture•Extended derivative ratio developed for this combination•These methods are sensitive and resolve mixtures with severely overlapping spectra.•The good recovery and accuracy make them applicable in QC laboratories.

New accurate, sensitive and selective spectrophotometric and chemometric methods were developed and subsequently validated for determination of Imipenem (IMP), ciprofloxacin hydrochloride (CIPRO), dexamethasone sodium phosphate (DEX), paracetamol (PAR) and cilastatin sodium (CIL) in human urine.These methods include a new derivative ratio method, namely extended derivative ratio (EDR), principal component regression (PCR) and partial least-squares (PLS) methods.A novel EDR method was developed for the determination of these drugs, where each component in the mixture was determined by using a mixture of the other four components as divisor. Peak amplitudes were recorded at 293.0 nm, 284.0 nm, 276.0 nm, 257.0 nm and 221.0 nm within linear concentration ranges 3.00–45.00, 1.00–15.00, 4.00–40.00, 1.50–25.00 and 4.00–50.00 μg mL− 1 for IMP, CIPRO, DEX, PAR and CIL, respectively.PCR and PLS-2 models were established for simultaneous determination of the studied drugs in the range of 3.00–15.00, 1.00–13.00, 4.00–12.00, 1.50–9.50, and 4.00–12.00 μg mL− 1 for IMP, CIPRO, DEX, PAR and CIL, respectively, by using eighteen mixtures as calibration set and seven mixtures as validation set.The suggested methods were validated according to the International Conference of Harmonization (ICH) guidelines and the results revealed that they were accurate, precise and reproducible. The obtained results were statistically compared with those of the published methods and there was no significant difference.

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