Article ID Journal Published Year Pages File Type
1229763 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2016 7 Pages PDF
Abstract

•Interaction of lysozyme with AgTiO2 nanoparticles was analyzed.•Static type of quenching is observed from fluorescence measurements.•Absorption and lifetime measurements confirm the static type quenching.•The presence of nanoparticles decreases the binding capability of drug with protein.•Present findings will provide vital informations for drug carrying nanoparticles.

Binding of lysozyme with AgTiO2 nanoparticles was analyzed by using absorption, fluorescence, time resolved and synchronous fluorescence measurements. In the presence of AgTiO2 nanoparticles, the fluorescence intensity of lysozyme was decreased. Static type of binding was confirmed through lifetime and ground state absorption measurements. From the fluorescence quenching data, the binding constant and the number of binding sites were found to be 1.5 × 104 M−1 and 1.03, respectively. From the synchronous fluorescence spectroscopic measurements, tryptophan residue in lysozyme was found to have interaction with the nanoparticles. Further, the influence of AgTiO2 nanoparticles on the binding strength of lysozyme with a drug molecule was analyzed through fluorescence quenching methods. The presence of nanoparticles decreases the binding capability of drug with protein. Overall, the observed results will provide basic insights on the utilization of nanoparticles in drug delivery applications.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , ,