Development a novel approach of chemiluminescent probe array

•A novel CL probe array assay was first developed.•It gives rapid and high throughput detection.•It breaks traditional development view in solid phase supports.

A new chemiluminescent (CL) probe array assay approach was first developed. The new CL probe array was based on Co3O4–SiO2 mesoporous nanocomposite material, which not only has an excellent catalytic effect on the luminol–H2O2 CL reaction in alkaline medium but also can be used for the immobilization of enzymes. As a model, the novel bifunctional CL probe array has been applied to the high-throughput determination of glucose in human. The linear range of the glucose concentration was 3–90 μM and the detection limit was 0.36 μM. It breaks traditional development view in solid phase supports and provides new insights into the application of mesoporous material.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide