| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1231152 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2012 | 5 Pages |
The interaction of proteins with quantum dots is an interesting field of research. These interactions occur at the nanoscale. We have probed the interaction of Bovine Serum Albumin (BSA) and Candida rugosa lipase (CRL) with rhamnolipid capped ZnS (RhlZnSQDs) using absorption and fluorescence spectroscopy. Optical studies on mixtures of RhlZnSQDs and proteins resulted in Förster’s Resonance Energy Transfer (FRET) from proteins to QDs. This phenomenon has been exploited to detect proteins in agarose gel electrophoresis. The activity of the CRL was unaffected on the addition of QDs as revealed by zymography.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Rhamnolipid capped ZnS Quantum dots (RhlZnSQDs). ► Interaction of RhlZnSQDs with proteins. ► Staining of proteins in agarose gels using RhlZnSQDs. ► RhlZnSQD and activity of proteins.
