| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1231426 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2012 | 7 Pages |
ZnO/hydroxylapatite (ZnO/HA) composite with HA molar content of 6.0% was prepared by the method of precipitation and heat-treated at 500 °C for 40 min and was characterized by powder X-ray diffraction (XRD). The sonocatalytic activities of ZnO/HA composite was carried out through the damage of bovine serum albumin (BSA) in aqueous solution. Furthermore, the effects of several factors on the damage of BSA molecules were evaluated by means of UV–vis and fluorescence spectra. Experimental results indicated that the damage degree of BSA aggravated with the increase of ultrasonic irradiation time, irradiation power and ZnO/HA addition amount, but weakened with the increase of solution acidity and ionic strength. In addition, the damage site to BSA was also studied by synchronous fluorescence technology and the damage site was mainly at tryptophan (Trp) residue. This paper provides a valuable reference for driving sonocatalytic method to treat tumor in clinic application.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► ZnO/HA were used as sonocatalyst to study the sonocatalytic damage of BSA. ► The effects of several factors on the sonocatalytic damage of BSA are studied. ► Synchronous fluorescence spectroscopy is used to study the damage site of BSA.
