| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1231506 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2013 | 11 Pages |
The objectives of this study were (i) to determine lipid related molecular structures components (functional groups) in feed combination of cereal grain (barley, Hordeum vulgare) and wheat (Triticum aestivum) based dried distillers grain solubles (wheat DDGSs) from bioethanol processing at five different combination ratios using univariate and multivariate molecular spectral analyses with infrared Fourier transform molecular spectroscopy, and (ii) to correlate lipid-related molecular-functional structure spectral profile to nutrient profiles. The spectral intensity of (i) CH3 asymmetric, CH2 asymmetric, CH3 symmetric and CH2 symmetric groups, (ii) unsaturation (CC) group, and (iii) carbonyl ester (CO) group were determined. Spectral differences of functional groups were detected by hierarchical cluster analysis (HCA) and principal components analysis (PCA). The results showed that the combination treatments significantly inflicted modifications (P < 0.05) in nutrient profile and lipid related molecular spectral intensity (CH2 asymmetric stretching peak height, CH2 symmetric stretching peak height, ratio of CH2 to CH3 symmetric stretching peak intensity, and carbonyl peak area). Ratio of CH2 to CH3 symmetric stretching peak intensity, and carbonyl peak significantly correlated with nutrient profiles. Both PCA and HCA differentiated lipid-related spectrum. In conclusion, the changes of lipid molecular structure spectral profiles through feed combination could be detected using molecular spectroscopy. These changes were associated with nutrient profiles and functionality.
Graphical abstractTypical lipid-related spectra regions (offset scale) in feed and food mixtures.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Feed combination affects lipid related molecular spectral intensity. ► PCA analyses are unable to distinct treatment differences. ► HCA differentiated lipid-related molecular spectral difference. ► The changes of lipid molecular structure are associated with nutrient functionality.
