The investigation of the interaction between Oxymetazoline hydrochloride and mucin by spectroscopic approaches

The fluorescence and ultraviolet spectroscopy were explored to study the interaction between Oxymetazoline hydrochloride (OMZH) and mucin under imitated physiological condition. The results demonstrated that the fluorescence quenching mechanism between OMZH and mucin is a combined quenching process. The binding constants (Ka), binding sites (n) and the corresponding thermodynamic parameters (ΔG, ΔH, and ΔS) of the interaction system were calculated at different temperatures. The hydrogen bonds and van der Waals forces play a major role in the interaction between OMZH and mucin. According to Förster non-radiation energy transfer theory, the binding distance between OMZH and mucin was calculated.

Graphical abstractThe interaction between Oxymetazoline hydrochloride (OMZH) and mucin was studied by fluorescence and UV–Vis spectroscopy. The quenching mechanism, binding constants and binding distance were obtained. The binding constants and binding sites were calculated. According to Förster non-radiation energy transfer theory, the binding distance between OMZH and mucin was calculated.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We explored the interaction of mucin and OMZH by spectroscopic methods. ► The fluorescence quenching mechanism is combined quenching. ► The binding constants and binding sites were calculated. ► Hydrogen bonds and van der Waals forces plays a major role in the interaction. ► The energy transfer between OMZH and mucin with high probability.