Interaction of drug based copper(II) complexes with Herring Sperm DNA and their biological activities

Square pyramidal Cu(II) complexes with NS donor ligand and ciprofloxacin have been synthesized and characterized using analytical and spectral techniques. The synthesized complexes have been tested for their antimicrobial activity using double dilution technique in terms of minimum inhibitory concentration (MIC) and colony forming unit (CFU). The DNA binding ability of the complexes with Sperm Herring DNA has been performed using absorption titration and viscosity measurement. The nuclease activity of complexes with plasmid DNA (pUC19) has been carried out using agarose gel electrophoresis technique. Synthesized complexes have been tested for their SOD mimic activity using NBT/NADH/PMS system. The cytotoxic properties of metal complexes have been evaluated using brine shrimp lethality bioassay.

Graphical abstractThe DNA binding ability of the complexes with Herring Sperm DNA has been performed using absorption titration. With increase in DNA to complex ratio, hypochromism and red shift was observed. It suggests that complexes bind via interaction mode.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Synthesis of substituted NS donor ligand and their Cu(II) complexes. ► Characterization by elemental analysis, IR, UV–visible, LC–MS spectrometry. ► All Cu(II) complexes have tested for their biological studies. ► The interaction of DNA with complexes has been performed.