Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1232303 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2012 | 6 Pages |
Abstract
⺠Static quenching is the main fluorescence quenching mechanism between Lys and BBR, and there is one binding site in Lys for BBR. ⺠BBR neutral radical react with Trp (K = 3.4 Ã 109 Mâ1 sâ1) via electron transfer to give the radical cation (Trp/NH
- +) and neutral radical (TrpN
- ) of Trp. ⺠BBR can selectively oxidize the Trp residues of Lys with the rate constant 4.2 Ã 108 Mâ1 sâ1. ⺠Through thermodynamic calculation, the reaction mechanisms between 3BBRâ and Trp or Lys were determined to be electron transfer process.
- +) and neutral radical (TrpN
- ) of Trp. ⺠BBR can selectively oxidize the Trp residues of Lys with the rate constant 4.2 Ã 108 Mâ1 sâ1. ⺠Through thermodynamic calculation, the reaction mechanisms between 3BBRâ and Trp or Lys were determined to be electron transfer process.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ling-Li Cheng, Mei Wang, Ming-Hong Wu, Si-De Yao, Zheng Jiao, Shi-Long Wang,