Distinction of broken cellular wall Ganoderma lucidum spores and G. lucidum spores using FTIR microspectroscopy

In this paper, FTIR microspectroscopy was used to identify broken cellular wall Ganoderma lucidum spores and G. lucidum spores. For IR spectra, broken cellular wall G. lucidum spores and G. lucidum spores were mainly different in the regions of 3000–2800, 1660–1600, 1400–1200 and 1100–1000 cm−1. For curve fitting, the results showed the differences in the protein secondary structures and the polysaccharide structures/content between broken cellular wall G. lucidum spores and G. lucidum spores. Moreover, the value of A1078/A1741 might be a potentially useful factor to distinguish broken cellular wall G. lucidum spores from G. lucidum spores. Additionally, FTIR microspectroscopy could identify broken cellular wall G. lucidum spores and G. lucidum spores accurately when it was combined with hierarchical cluster analysis. The result suggests FTIR microspectroscopy is very simple and efficient for distinction of broken cellular wall G. lucidum spores and G. lucidum spores. The result also indicates FTIR microspectroscopy may be useful for TCM identification.

Graphical abstractIn this paper, FTIR microspectroscopy was used to identify broken cellular wall Ganoderma lucidum spores (A) and G. lucidum spores (B).Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Broken and unbroken cellular wall Ganoderma lucidum spores were studied by FTIR microspectroscopy. ► IR spectra of the above samples were compared. ► Curve fitting was used to study differences of the above samples. ► A1640/A1741, A1078/A1640, A1078/A1741 values of the above samples were compared. ► FTIR microspectroscopy could identify the above samples accurately.