| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1232772 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2012 | 5 Pages |
With alkaline phosphatase (ALP)–adamantane (AMPPD) system as the chemiluminescence (CL) detection system, a highly sensitive, specific and simple competitive chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of enrofloxacin (ENR). The physicochemical parameters, such as the chemiluminescent assay mediums, the dilution buffer of ENR–McAb, the volume of dilution buffer, the monoclonal antibody concentration, the incubation time, and other relevant variables of the immunoassay have been optimized. Under the optimal conditions, the detection linear range of 350–1000 pg/mL and the detection limit of 0.24 ng/mL were provided by the proposed method. The relative standard deviations were less than 15% for both intra and inter-assay precision. This method has been successfully applied to determine ENR in spiked samples with the recovery of 103%–96%. It showed that CLEIA was a good potential method in the analysis of residues of veterinary drugs after treatment of related diseases.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A highly sensitive and specific CLEIA using ALP–AMPPD system was developed for the determination of ENR residue. ► The limit of detection was 0.24 ng/mL with the minimum amount of ENR in comparison with HPLC and ELISA. ► The method expressed good application potential in the analysis of ENR in food.
