FT-IR spectral studies on certain human urinary stones in the patients of rural area

Fourier transform infrared spectroscopy (FT-IR) has been carried out to analyze the organic and inorganic constituent of human urinary stones. Patient's hailing from Rajah Muthiah Medical College and Hospital, Annamalai University, Tamil Nadu, India was selected for the study. The FT-IR results indicate that stones have different composition, i.e., namely calcium oxalate, calcium phosphate, carbonate apatite and magnesium ammonium phosphate and uric acid. From the spectral and powder X-ray diffraction pattern, the chemical constituents of urinary stones were identified. The quantitative estimations of calcium oxalate monohydrate (COM) 1620 cm−1, calcium phosphate (apatite) 1037 cm−1, magnesium ammonium phosphate (struvite) 1010 cm−1, calcium carbonate 1460 cm−1 and uric acid 1441 cm−1 were calculated using particular peaks of FT-IR studies. The study reveals that calcium oxalate monohydrate and calcium phosphate type urinary stones were predominant whereas magnesium ammonium phosphate are in moderate level, and calcium carbonate and uric acid are in low. Calcium phosphate is found in all the stones and calcium oxalate monohydrate is found to be higher. Quantitative analyses of urinary stones show that calcium oxalate monohydrate (40%), apatite (30%), magnesium ammonium phosphate (23%) and uric acid (7%) are present in all the urinary stone samples.

Graphical abstractFT-IR and power X-ray study results indicate that, stones have different composition, i.e., calcium oxalate, calcium phosphate, carbonate apatite and magnesium ammonium phosphate and uric acid. From the spectral, the chemical constituents of urinary stones were identified. The quantitative estimation was performed for four consecutive peaks for calcium oxalate monohydrate (COM), calcium phosphate (apatite), Struvite (MAPH) and uric acid.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► FT-IR spectra of twenty human urinary stones were analysed. ► The chemical constituents of human urinary stones were identified. ► Qualitative and quantitative estimation of human urinary stones were performed.