Bioinspired synthesis of highly stabilized silver nanoparticles using Ocimum tenuiflorum leaf extract and their antibacterial activity

Biosynthesis of nanoparticles is under exploration due to wide biomedical applications and research interest in nanotechnology. We herein reports bioinspired synthesis of silver nanoparticles with the aid of novel, non toxic ecofriendly biological material namely Ocimum tenuiflorum leaf extract. It acts as reducing as well as stabilizing agent. An intense surface plasmon resonance band at ∼450 nm in the UV–visible spectrum clearly reveals the formation of silver nanoparticles. The photoluminescence spectrum was recorded to study excitation and emission. TEM and PSD by dynamic light scattering studies showed that size of silver nanoparticles to be in range 25–40 nm. Face centered cubic structure of silver nanoparticles are confirmed by SAED pattern. The charge on synthesized silver nanoparticles was determined by zeta potential. The colloidal solution of silver nanoparticles were found to exhibit high antibacterial activity against three different strains of bacteria Escherichia coli (Gram negative), Corney bacterium (gram positive), Bacillus substilus (spore forming).

Graphical abstractTEM-image of bioinspired silver nanoparticles.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Synthesis of SNPs with novel, non toxic, ecofriendly material is reported. ► Method is simple, cost effective, and stable for long time and reproducible to obtain stable SNPs. ► The bioinspired SNPs show high antibacterial activity towards various bacterial strains. ► The results of UV–visible, TEM and PSD are good agreement. ► Bioinspired SNPs are very important because of their applications in biomedical fields.