Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1233062 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2009 | 7 Pages |
Abstract
Fluorescein (HFin) emitted strong and stable room temperature phosphorescence (RTP) on filter paper after set at 50 °C for 10 min using Li+ as the ion perturber. HFin existed as Finâ when the pH value was in the range of 5.45-7.36. Finâ could react with [Cu(BPY)2]2+ (BPY: α,α-bipyridyl) to produce ion association complex [Cu(BPY)2]2+·[(Fin)2]2â, which could enhance the RTP signal of Hfin. In the presence of bovine serum albumin (BSA), the -COOH group of Finâ in the [Cu(BPY)2]2+·[(Fin)2]2â could react with the -NH2 group of BSA to form the ion association complex [Cu(BPY)2]2+·[(Fin-BSA)2]2â, which contained -CO-NH- bond. This complex could sharply enhance the RTP signal of Hfin and the ÎIp was directly proportional to the content of BSA. According to the facts above, a new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace protein had been established using the ion association complex [Cu(BPY)2]2+·[(Fin)2]2âas a phosphorescent probe. This method had wide linear range (0.40 Ã 10â9-280 Ã 10â9 mg lâ1), high sensitivity (the detection limit (LD) was 1.4 Ã 10â10 mg lâ1), good precision (RSD: 3.4-4.9%) and high selectivity (the allowed concentration of coexistent ions or coexistent materials was high). It had been applied to the determination of the content of protein in 10 kinds of real samples, and the result agreed well with pyrocatechol violet-Mo (VI) method (P.V.M.M.), which indicated it had high accuracy. Meanwhile, reaction mechanism for the determination of trace protein with [Cu(BPY)2]2+·[(Fin)2]2â phosphorescent probe was also discussed. The academic thought of this research could not only be used to develop many kinds of ion association complex phosphorescent probes, but also provided a new way to promote the sensitivity of SS-RTP.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jia-Ming Liu, Shao-Qin Lin, Chang-Qing Lin, Li-Qun Chen, Fei-Ming Li, Li-Qing Zeng, Xiao-Mei Huang,