Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1233072 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2009 | 4 Pages |
Abstract
In this paper, two flavonoid aglycones (baicalein, quercetin) and their glycosides (baicalin, quercitrin) were studied for their ability to bind protein by quenching the protein intrinsic fluorescence. From the spectra obtained, the bimolecular quenching constants, the apparent static binding constants, and binding sites values were calculated. The glycosylation of flavonoids decreases the binding affinity with protein. For quercetin and quercitrin, the binding constants for BSA were 3.65 × 107 and 6.47 × 103 L mol−1, respectively. For baicalein and baicalin, the binding constants were 4.54 × 108 and 1.63 × 106 L mol−1, respectively.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Hui Cao, Donghui Wu, Hongxian Wang, Ming Xu,