| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1233298 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2011 | 6 Pages |
The binding properties of amino phosphate ester derivatives, compound 1 and 2 with calf thymus DNA (CT-DNA) were investigated by UV spectra, fluorescence spectra, molecular modeling and isothermal titration calorimetry (ITC). The intrinsic binding constants Kb of compound 1 and 2 with CT-DNA were determined by fluorescence spectroscopy and ITC, respectively. The results indicated that the two compounds bind to CT-DNA with different binding affinity, which is in the order of compound 1 > compound 2. At the same time, fluorescence spectra suggested that the mechanism of the binding of the two compounds to CT-DNA is a static enhancing type. According to the ethidium bromide displacement experiments, UV spectra, molecular modeling and ITC studies, it can be concluded that compound 1 and 2 are intercalators that can slide into the G–C rich region of CT-DNA. Furthermore, ITC data showed that compound/DNA binding is enthalpy controlled.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The two compounds can intercalate the DNA base pairs. ► The interaction mechanism of two compounds to CT-DNA was static enhancing type. ► Compound/DNA binding is enthalpy controlled. ► Compounds can slide into the G–C rich region of CT-DNA.
