Article ID Journal Published Year Pages File Type
1233305 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2011 7 Pages PDF
Abstract

A benzimidazole derivative, 1-(2-picolyl)-3-(2-picolyl) benzimidazole iodide (PPB), was synthesized. Fourier transform infrared spectroscopy (FT-IR), UV–visible, three-dimensional (3D) fluorescence, synchronous fluorescence (SF) and fluorescence spectroscopic methods were used to determine the PPB binding mode and the effects of PPB on protein stability and secondary structure. Fluorescence results revealed the presence of static type of quenching mechanism in the binding of PPB to human serum albumin (HSA). The binding constants between PPB and HSA were obtained according to Scatchard equation. The number of binding sites, the binding constants and the thermodynamic parameters were measured. The results showed a spontaneous binding of PPB to HSA through hydrogen bonds and van der Waals forces. In addition, the distance between PPB and the Trp 214 was estimated via employing the Förster's non-radiative energy transfer theory, and was found to be 3.49 nm, which indicated that PPB can bind to HSA with high probability. Site marker competitive experiments indicated that the binding of PPB to HSA primarily took place in subdomain IIA.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► A novel benzimidazoles derivative, 1-(2-picolyl)-3-(2-picolyl) benzimidazole iodide (PPB), has been synthesized. ► Binding behavior of PPB with HSA was investigated under simulated physiologic conditions. ► The specific binding site of PPB on HSA was investigated by displacement experiments.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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