| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1233325 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2011 | 7 Pages |
Narrowly distributed cationic poly (methyl methacrylate-co-diacetone acrylamide) (P(MMA-DAAM)) nanoparticles were successfully prepared by microemulsion polymerization. Photon correlation spectrometer (PCS) measurement and transmission electron microscope (TEM) observation revealed that z-average particle size of P(MMA-DAAM) is ∼27.5 nm. It was found that these cationic nanoparticles interact with DNA through electrostatic interaction to form P(MMA-DAAM)–DNA complex, which significantly enhances the resonance light scattering (RLS) signal. Therefore, a novel method using this polymer nanoparticle as a new probe for the detection of DNA by RLS technique is developed in this paper. The results showed this method is very convenient, sensitive, and reproducible.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► P(MMA-DAAM) interact with DNA through electrostatic interaction to form complex. ► Interaction cause obvious change in size, morphology and zeta potential of complex. ► The formation of P(MMA-DAAM)-DNA complex significantly enhances the RLS signal. ► Some co-existing species had little effects on the determination of DNA. ► Our approach for DNA detection has a low determination limit (3σ) of 10.01ng mL-1.
