Comparative study between univariate spectrophotometry and multivariate calibration as analytical tools for simultaneous quantitation of Moexipril and Hydrochlorothiazide

•Comparison between univariate spectrophotometry and multivariate calibration.•The reported methods need sophisticated and expensive instruments and reagents.•First chemometric methods done for this combination.•The good recovery and accuracy makes it applicable in quality control laboratories.

Three simple, accurate, reproducible, and selective methods have been developed and subsequently validated for the simultaneous determination of Moexipril (MOX) and Hydrochlorothiazide (HCTZ) in pharmaceutical dosage form. The first method is the new extended ratio subtraction method (EXRSM) coupled to ratio subtraction method (RSM) for determination of both drugs in commercial dosage form. The second and third methods are multivariate calibration which include Principal Component Regression (PCR) and Partial Least Squares (PLSs). A detailed validation of the methods was performed following the ICH guidelines and the standard curves were found to be linear in the range of 10–60 and 2–30 for MOX and HCTZ in EXRSM method, respectively, with well accepted mean correlation coefficient for each analyte. The intra-day and inter-day precision and accuracy results were well within the acceptable limits.

Graphical abstractZero order absorption spectra of 30 μg/mL MOX (......), and 10 μg/mL HCTZ (—) showing severe overlapping.Figure optionsDownload full-size imageDownload as PowerPoint slide