| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1233495 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2014 | 6 Pages |
•The drawbacks of traditional CPE problems are overcome by dual-cloud point extraction.•The effects of the surfactant on the analysis and separation of analytes are eliminated.•The developed d-CPE was applied to evaluate Cu in serum samples of viral hepatitis patients.•The hepatitis patients of B, C and D have high levels of Cu in serum than patients have A and E virus.
An efficient, innovative preconcentration method, dual-cloud point extraction (d-CPE) has been developed for the extraction and preconcentration of copper (Cu2+) in serum samples of different viral hepatitis patients prior to couple with flame atomic absorption spectrometry (FAAS). The d-CPE procedure was based on forming complexes of elemental ions with complexing reagent 1-(2-pyridylazo)-2-naphthol (PAN), and subsequent entrapping the complexes in nonionic surfactant (Triton X-114). Then the surfactant rich phase containing the metal complexes was treated with aqueous nitric acid solution, and metal ions were back extracted into the aqueous phase, as second cloud point extraction stage, and finally determined by flame atomic absorption spectrometry using conventional nebulization. The multivariate strategy was applied to estimate the optimum values of experimental variables for the recovery of Cu2+ using d-CPE. In optimum experimental conditions, the limit of detection and the enrichment factor were 0.046 μg L−1 and 78, respectively. The validity and accuracy of proposed method were checked by analysis of Cu2+ in certified sample of serum (CRM) by d-CPE and conventional CPE procedure on same CRM. The proposed method was successfully applied to the determination of Cu2+ in serum samples of different viral hepatitis patients and healthy controls.
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