| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1233638 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2009 | 5 Pages |
In the present paper we report the results obtained with the photosystem II (PSII) isolated from spinach treated by MgCl2, and studied the effect of Mg2+ on spectral characteristics and photosynthetic functions of PSII. The results showed that Mg2+ treatment at a suitable concentration could significantly increase the absorption intensity of PSII and the intensity ratio of Soret band to Q band of chlorophyll-a. The treatment also elevated the excited peak intensity at 230, 278 and 343 nm, and the emitted peak intensity at 304 and 682 nm, and the ratio of F278/F230, respectively. The results implied that Mg2+ increased absorbance for visible light, improving energy transfer among amino acids within PSII protein complex and accelerating energy transport from tyrosine residue to chlorophyll-a. The photochemical activity and oxygen evolving rate of PSII were also enhanced by Mg2+. This is viewed as evidence that Mg2+ can promote energy transfer and oxygen evolution in PSII of spinach.
