Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1233673 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2010 | 5 Pages |
Abstract
We here describe a new spectrophotometric method for measuring total bilirubin in serum. The method is based on the cleavage of bilirubin giving formaldehyde which further reacts with diazotized 3-methyl-2-benzothiazolinone hydrazone hydrochloride giving blue colored solution with maximum absorbance at 630 nm. Sensitivity of the developed method was compared with Jendrassik-Grof assay procedure and its applicability has been tested with human serum samples. Good correlation was attained between both methods giving slope of 0.994, intercept 0.015, and R2 = 0.997. Beers law obeyed in the range of 0.068-17.2 μM with good linearity, absorbance y = 0.044Cbil + 0.003. Relative standard deviation was 0.006872, within day precision ranged 0.3-1.2% and day-to-day precision ranged 1-6%. Recovery of the method varied from 97 to 102%. The proposed method has higher sensitivity with less interference. The obtained product was extracted and was spectrally characterized for structural confirmation with FT-IR, 1H NMR.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Padmarajaiah Nagaraja, Krishnegowda Avinash, Anantharaman Shivakumar, Rangappa Dinesh, Ashwinee Kumar Shrestha,