Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1235307 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2008 | 6 Pages |
Abstract
Increasing evidence suggests that S-nitrosothiols (RSNO) may represent naturally occurring nitric oxide (NO) surrogates and function as intermediates in NO metabolism. In this work, a simple, sensitive, and selective micromethod is developed and validated for quantification of RSNO. A fluorescent probe 8-(3â²,4â²-diaminophenyl)-difluoroboradiaza-s-indacence (DABODIPY) is firstly used to label RSNO. The derivatization reaction is performed in aqueous medium at 30 °C for 15 min in the presence of 6 Ã 10â5 mol Lâ1 Hg2+ and the derivative is detected by fluorescence at λex/λem = 500/510 nm. A linear function of concentration in the range of (2.0-600.0) Ã 10â8 mol Lâ1 is observed with a correlation coefficient of 0.9992 and detection limit of 1.2 Ã 10â9 mol Lâ1 (S/N = 3). This technique has been successfully applied to quantify RSNO in some human blood samples including healthy persons and patients suffering from cardiovascular diseases.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ke-Jing Huang, Chun-Xuan Xu, Wan-Zhen Xie, Hua-Shan Zhang, Hong Wang,