Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1235788 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2007 | 5 Pages |
Abstract
Scopoletine (SLT), 7-hydroxy-6-methoxylcoumarin, is known to possess biological activities such as abirritating and anti-tumor, it can quench intrinsic fluorescence of bovine serum albumin (BSA) and the fluorescence intensity of itself is enhanced. So, SLT is used as fluorescence probe for quantitative determination of protein. The experiments indicate that under optimum conditions, the enhanced intensity of fluorescence is in proportion to the concentration of proteins in a wide range, and their detection limits (S/N = 3) are 1.4 × 10−8 g mL−1 for BSA and 1.1 × 10−8 g mL−1 for HSA, respectively. Samples were satisfactorily determined. The interaction mechanism is also discussed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yanjing Chen, Jinghe Yang, Zhiling Wang, Xia Wu, Fei Wang,